PUBLICATIONS
Lehmann, M., Noack, D., Wood, M., Perego, M., Knaus, U. 2009. Lung epithelial injugy by B. anthracis lethal toxin is caused by MKK-dependent loss of cytoskeletal integrity. PLoS One. March. 4(3):e4755 [Epub ahead of print]
Wilson, A.C., Hoch, J.A., Perego, M. 2009. Two small c-type cytochromes affect virulence gene expression in Bacillus anthracis. Mol. Microbiol. Feb 13. [Epub ahead of print]
Weigt, M., White, R.A., Szurmant, H., Hoch, J.A., Hwa, T. 2009. Identification of direct residue contacts in protein-protein interaction by message passing. Proc. Natl. Acad. Sci. USA. 106(1):67-72.
Scaramozzino, F., White, A., Perego, M., Hoch, J.A. 2009. A unique GTP-dependent sporulation sensor histidine kinase in Bacillus anthracis. J Bacteriol. 191(3):687-92.
Wilson, A.C., Soyer, M., Hoch, J.A., Perego, M. 2008. The bicarbonate transporter is essential for Bacillus anthracis lethality. PLoS Pathog. 4(11):e1000210.
Del Papa, M.F. and Perego, M. 2008. Ethanolamine activates a sensor histidine kinase regulating its utilization in Enterococcus faecalis. J. Bacteriol. 190(21):7147-56.
Bongiorni, C., Fukushima, T., Wilson, A.C., Chiang, C., Mansilla, M.C., Hoch, J.A. and Perego, M. 2008. Dual promoters control the expression of the Bacillus anthracis virulence factor AtxA. J Bacteriol. Oct;190(19):6483-92.
Fukushima, T., Szurmant, H., Kim, E.-J., Perego, M. and Hoch, J.A. 2008. A sensor histidine kinase coordinates cell wall architecture with cell division in Bacillus subtilis. Mol. Microbiol. 69(3):621-32.
Szurmant, H., Bobay, B.G., White, R.A., Sullivan, D.M., Thompson, R.J., Hwa, T., Hoch, J.A. and Cavanagh, J. 2008. Co-evolving motions at protein-protein interfaces of two-component signaling systems identified by covariance analysis. Biochemistry. 47(30):7782-4.
Wilson, A.C., Hoch, J.A. and Perego, M. 2008. Virulence gene expression is independent of ResDE-regulated respiration control in Bacillus anthracis. J. Bacteriol. 190(15):5522-5.
Perego, M. and Hoch, J.A. 2008. Commingling regulatory systems following acquisition of virulence plasmids by Bacillus anthracis. Trends Microbiol. 16(5):215-21.
Szurmant, H., Bu, L., Brooks III, C.L. and Hoch, J.A. 2008. An essential sensor histidine kinase controlled by transmembrane helix interactions with its auxiliary proteins. Proc Natl Acad Sci . 105(15):5891-6.
Winkler, M.E. and Hoch, J.A. 2008. Essentiality, bypass, and targeting of the YycFG (VicRK) two-component regulatory system in Gram-positive bacteria. J. Bacteriol.190(8):2645-8.
Szurmant, H., White, R.A. and Hoch, J.A. 2007. Sensor complexes regulating two-component signal transduction. Curr. Opin. Struct. Biol. 17(6):706-15.
Diaz, A.R., Stephenson, S., Green, J.M., Levdikov, V.M., Wilkinson, A.J. and Perego, M. 2007. Functional role for a conserved aspartate in the Spo0E signature motif involved in the dephosphorylation of the Bacillus subtilis sporulation regulator Spo0A. J Biol. Chem. 283(5):2962-72.
Del Papa, M.F., Hancock, L., Thomas, V. and Perego, M. 2007. Full activation of Enterococcus faecalis gelatinase by a C-terminal proteolytic cleavage. J. Bacteriol.189(24):8835-43.
Wilson, A.C., Perego, M. and Hoch, J.A. 2007. New transposon delivery plasmids for insertional mutagenesis in Bacillus anthracis. J. Microbiol. Methods.71(3):332-5.
Szurmant, H., Fukushima, T. and Hoch, J.A. 2007. The Essential YycFG Two-Component System of Bacillus subtilis. Methods Enzymol. 422:396-417.
White, R.A., Szurmant, H., Hoch, J.A. and Hwa, T. 2007. Features of protein-protein interactions in two-component signaling deduced from genomic libraries. Methods Enzymol. 422:75-101.
McLaughlin, P.D., Bobay, B.G., Regel, E.J., Thompson, R.J., Hoch, J.A. and Cavanagh, J. 2007. Predominantly buried residues in the response regulator Spo0F influence specific sensor kinase recognition. FEBS Lett. 581(7):1425-9.
Szurmant, H., Mohan, M.A., Imus, P.M. and Hoch, J.A. 2007. YycH and YycI interact to regulate the essential YycFG two-component system in Bacillus subtilis. J. Bacteriol. 189(8):3280-9.
Santelli, E., Liddington, R.C, Mohan, M.A., Hoch, J.A. and Szurmant, H. 2007. The crystal structure of Bacillus subtilis YycI reveals a common fold for two members of an unusual class of sensor histidine kinase regulatory proteins. J. Bacteriol. 189(8):3290-5.
Smits, W.K., Bongiorni, C., Veening, J.W., Hamoen, L.W., Kuipers, O.P. and Perego, M. 2007. Temporal separation of distinct differentiation pathways by a dual specificity Rap-Phr system in Bacillus subtilis. Mol. Microbiol. 65(1):103-20.
Bongiorni, C., Stoessel, R. and Perego, M. 2007. Negative regulation of Bacillus anthracis sporulation by the Spo0E family of phosphatases. J. Bacteriol. 189(7):2637-45.
Tsvetanova, B., Wilson, A.C., Bongiorni, C., Chiang, C., Hoch, J.A. and Perego, M. 2007. Opposing effects of histidine phosphorylation regulate the AtxA virulence transcription factor in Bacillus anthracis. Mol. Microbio. 63(3):644-55.
Grenha, R., Rzechorzek, N.J., Brannigan, J.A., de Jong, R.N., Ab, E., Diercks, T., Truffault, V., Ladds, J.C., Fogg, M.J., Bongiorni, C., Perego, M., Kaptein, R., Wilson, K.S., Folkers, G.E. and Wilkinson, A.J. 2006. Structural characterization of Spo0E-like protein aspartic acid phosphatases that regulate sporulation in Bacilli. J. Biol. Chem. 218(49):37993-8003.
White, A.K., Hoch, J.A., Grynberg, A., Godzik, A. and Perego, M. 2006. Sensor domains encoded in Bacillus anthracis virulence plasmids prevent sporulation by hijacking a sporulation sensor histidine kinase. J. Bacteriol. 188(17):6354-60
Szurmant, H., Zhao, H., Mohan, M.A., Hoch, J.A. and Varughese K.I. 2006. The Crystal structure of YycH involved in the regulation of the essential YycFG two-component system in Bacillus subtilis reveals a novel tertiary structure. Protein Science 15(4):929-934
Worner, K., Szurmant, H., Chiang, C. and Hoch, J.A. 2006. Phosphorylation and Functional Analysis of the Sporulation Initiation Factor Spo0A from Clostridium botulinum. Mol. Microbiol. 59(3):1000-1012
Bongiorni, C., Stoessel, R., Shoemaker, D. and Perego M. 2006. Rap phosphatase of virulence plasmid pXO1 inhibits Bacillus anthracis sporulation. J. Bacteriol. 188(2):487-498
Kojetin, D.J., Thompson, R.J., Benson, L.M., Naylor, S., Waterman, J., Davies, K.G., Opperman, C.H., Stephenson, K., Hoch, J.A. and Cavanagh, J. 2005. Structural analysis of divalent binding to the Bacillus subtilis response regulator Spo0F: the possibility for in vitro metalloregulation in the initiation of sporulation. Biometals. 18(5):449-466
Brunsing, R.L., La Clair, C., Chiang, C., Hancock, L.E., Perego, M. and Hoch, J.A. 2005. Characterization of sporulation histidine kinases of Bacillus anthracis. J. Bacteriol. 187(20):6972-81.
Low, L.Y., Yang, C., Perego, M., Osterman, A. and Liddington, R.C. 2005. Structure and lytic activity of a bacillus anthracis prophage endolysin. J Biol Chem. 280(42):35433-9.
Szurmant, H., Nelson, K., Kim, E.J., Perego, M. and Hoch, J.A. 2005. YycH regulates the activity of the essential YycFG two-component system in Bacillus subtilis. J Bacteriol.187(15):5419-26.
Musumeci, L., Bongiorni, C., Tautz, L., Edwards, R.A., Osterman, A., Perego, M., Mustelin, T. and Bottini, N. 2005. Low-molecular-weight protein tyrosine phosphatases of Bacillus subtilis. J Bacteriol. 187(14):4945-56.
Bongiorni, C., Ishikawa, S., Stephenson, S., Ogasawara, N. andPerego, M. 2005. Synergistic regulation of competence development in Bacillus sub tilis by two Rap-Phr systems. J Bacteriol. 187(13):4353-61.
Hancock, L.E. and M. Perego. 2004. Systematic Inactivation and Phenotypic Characterization of Two-Component Signal Transduction Systems of Enterococcus faecalis V583. J. Bacteriol. 186(23):7951-7958.
Hancock, L.E. and M. Perego. 2004. The Enterococcus faecalis fsr Two-Component System Controls Biofilm Development through Production of Gelatinase. J. Bacteriol. 186(17):5629-39.
Kao, M.C,, Di Bernardo, S., Perego, M., Nakamaru-Ogiso, E., Matsuno-Yagi, A. and Yagi, T. 2004. Functional roles of four conserved charged residues in the membrane domain subunit NuoA of the proton-translocating NADH-quinone oxidoreductase from Escherichia coli. J Biol Chem. 279(31):32360-6.
Stephenson, K. and J.A. Hoch. 2004. Developing Inhibitors to Selectively Target Two-Component and Phosphorelay Signal Transduction Systems of Pathogenic Microorganisms. Curr. Med. Chem. 11(6):765-73
M. Perego, J.A. Hoch and Barrett, J.F. 2004. Functional Genomics of Gram-Positive Microorganisms. J. Bacteriol. 186(4):903-909
Core, L. and M. Perego. 2003. TPR-mediated interaction of RapC with ComA inhibits response regulator-DNA binding for competence development in Bacillus subtilis. Mol. Microbiol. 49(6):1509-1522
Stephenson, S., Mueller, C., Jiang, M. and M. Perego. 2003. Molecular analysis of Phr peptide processing in Bacillus subtilis. J. Bacteriol. 185(16):4861-1871
Stephenson, K. and J.A. Hoch. 2002. Histidine kinase-mediated signal transduction systems of pathogenic microorganisms as targets for therapeutic intervention. Curr. Drug Targets – Infectious Disorders. 2(3):235-246
Stephenson, K. and J.A. Hoch. 2002. Evolution of signaling in the sporulation phosphorelay. Mol. Microbiol. 46(2):297-304
Hancock, L. and M. Perego. 2002. Two-component signal transduction in Enterococcus faecalis. J. Bacteriol. 184(21):5819-5825
Stephenson, K. and J.A. Hoch. 2002. Two-component and phosphorelay signal transduction systems as therapeutic targets. Curr.Opin.Pharm. 2(5):507-512
Zhao, H., T. Msadek, J. Zapf, Madhusudan, J.A. Hoch and K.I. Varughese. 2002. DNA complexed structure of the key transcription factor initiating development in sporulating bacteria. Structure 10(8):1041-1050
Stephenson, K. and Hoch, J. A. 2002. Virulence- and antibiotic resistance-associated two-component signal transduction systems of Gram-positive pathogenic bacteria as targets for anti-microbial therapy. Pharmacology and Therapeutics 93(2-3):293-305
Stephenson, S.J. and M. Perego. 2002. Interaction surface of the Spo0A response regulator with the Spo0E phosphatase. Mol. Microbiol. 44(6):1455-1467
Ishikawa, S., Core, L.J. and M. Perego. 2002. Biochemical characterization of aspartyl phosphate phosphatase interaction with a phosphorylated response regulator and its inhibition by a pentapeptide. J. Biol. Chem. 277(23):20483-20489
Sievers, J., B. Raether, M. Perego and J. Errington. 2002. Characterization of the parB-like yyaA gene of Bacillus subtilis. J. Bacteriol. 184(4):1102-1111
Kanamaru, K., S. Stephenson and M. Perego. 2002. Overexpression of the PepF oligopeptidase inhibits sporulation initiation in Bacillus subtilis. J. Bacteriol. 184(1):43-50
Perego, M. and J.A. Hoch. 2002. Two-component systems, phosphorelays and regulation of their activities by phosphatases, pp.473-481. In A.L. Sonenshein, et. al. (ed.) Bacillus subtilis and Its Closest Relatives: From Genes to Cells. ASM Press, Washington, D.C.
Stephenson, K. and J.A. Hoch. 2001. PAS-A domain of phosphorelay sensor kinase A: A catalytic ATP-binding domain involved in the initiation of development in Bacillus subtilis. PNAS 98(26):15251-15256
Perego, M. and J.A. Hoch. 2001. Functional genomics of Gram-positive microorganisms: Review of the meeting, San Diego, California, 24 to 28 June 2001. J. Bacteriol. 183(24):6973-6978
Sen, U., Vasudevan, S., Subbarao, G., McClintock, R.A., Celikel, R., Ruggeri, Z.M. and K.I. Varughese. 2001. Crystal structure of the von Willebrand factor modulator botrocetin. Biochemistry 40(2):345-352
Perego, M. 2001. A new family of aspartyl phosphate phosphatases targeting the sporulation transcription factor Spo0A of Bacillus subtilis. Mol. Microbiol. 42(1):133-143
Perego, M. and J.A. Brannigan. 2001. Pentapeptide regulation of aspartyl-phosphate phosphatases. Peptides 22(10):1541-1547
Core, L.J., Ishikawa, S., and M. Perego. 2001. A free terminal carboxylate group is required for PhrA pentapeptide inhibition of RapA phosphatase. Peptides 22(10):1549-1553
Varughese, K.I. and J.A. Hoch. 2001. Keeping signals straight in phosphorelay signal transduction. J. Bacteriol. 183(17):4941-4949
Wang, M., Fabret, C., Kanamaru, K., Stephenson, K., Dartois, V., Perego, M., and J.A. Hoch. 2001. Dissection of the functional and structural domains of phosphorelay histidine kinase A of Bacillus subtilis. J. Bacteriol. 183(9):2795-2802
Celikel, R., Ruggeri, Z.M. and K.I. Varughese. 2000. von Willebrand factor conformation and adhesive function is modulated by an internalized water molecule. Nature Structural Biol. 7(10):881-884
Zapf, J., Sen, U., Madhusudan, Hoch, J.A., and K.I. Varughese. 2000. A transient interaction between two phosphorelay proteins trapped in a crystal lattice reveals the mechanism of molecular recognition and phosphotransfer in signal transduction. Structure 8(8):851-862
Jiang, M., Shao, W., Perego, M., and J.A. Hoch. 2000. Multiple histidine kinases regulate entry into stationary phase and sporulation in Bacillus subtilis. Mol. Microbiol. 38(3):535-542
Stephenson, K., Yamaguchi, Y., and J.A. Hoch. 2000. The Mechanism of Action of Inhibitors of Bacterial Two-component Signal Transduction Systems. J. Biol. Chem. 275(49):38900-38904
Jiang, M., Grau, R., and M. Perego. 2000. Differential processing of propeptide Inhibitors of Rap Phosphatases in Bacillus subtilis. J. Bacteriol. 182(2):303-310
J.A. Hoch. 2000. Two-component and Phosphorelay Signal Transduction. Curr. Opin. Microbiol. 3(2):165-170
Strauch, M.A. and J.A. Hoch. 1999. Spores, Sporulation, pp.2417-2421. In T.E. Creighton (ed.) The Encyclopedia of Molecular Biology. John Wiley & Sons, New York, NY.
Gilmore, M.S. and J.A. Hoch. 1999. Antibiotic Resistance: A Vancomycin surprise. Nature 399(6736):524-527
Koide, A., M. Perego and J.A. Hoch. 1999. ScoC Regulates Peptide Transport and Sporulation Initiation in Bacillus subtilis. J. Bacteriol. 181(13):4114-4117
Jiang, M., Y.-L. Tzeng, V.A. Feher, M. Perego and J.A. Hoch. 1999. Alanine Mutants of the Spo0F Response Regulator Modifying Specificity for Sensor Kinases in Sporulation Initiation. Mol. Microbiol. 33(2):389-395
Fabret, C., V.A. Feher and J.A. Hoch. 1999. Two-Component Signal Transduction In Bacillus subtilis: How One Organism Sees Its World. J. Bacteriol. 181(7):1975-1983
Perego, M. 1999. Self-signaling by Phr peptides modulate Bacillus subtilis development, pp. 243-258. In Cell-Cell signaling in Bacteria. G.M. Dunny, and S.C. Winans (Eds.) ASM Press, Washington, D.C.
Fabret, C. and J.A. Hoch. 1998. A Two-Component Signal Transduction System Essential for Growth of Bacillus subtilis: Implications for Anti-Infective Therapy. J. Bacteriol. 180(23):6375-6383
Varughese, K.I., Madusudan, X.Z. Zhou, J.M. Whiteley and J.A. Hoch. 1998. Formation of a Novel Four-Helix Bundle and Molecular Recognition Sites by Dimerization of a Response Regulator Phosphotransferase. Molec. Cell. 2(4):485-493
Tzeng, Y.-L., V.A. Feher, J. Cavanagh, M. Perego and J.A. Hoch. 1998. Characterization of interactions between a two-component response regulator, Spo0F, and its phosphatase, RapB. Biochemistry 37(47):16538-16545
Tzeng, Y.-L., X.Z. Zhou and J.A. Hoch. 1998. Phosphorylation of the Spo0B response regulator phosphotransferase initiating development in Bacillus subtilis. J. Biol. Chem. 273(37):23849-23855
Cervin, M.A., G.B. Spiegelman, B. Raether, K. Ohlsen, M. Perego and J.A. Hoch. 1998. A negative regulator linking chromosome segregation to developmental transcription in Bacillus subtilis. Mol. Microbiol. 29(1):85-95
Barrett, J.F. and J.A. Hoch. 1998. Two-component signal transduction as a target for microbial anti-infective therapy. Antimicrob. Agents. Chemother. 42(7):1529-1536
Hoch, J.A. 1998. Initiation of bacterial development. Current Opinion Microbiol. 1(2):170-174
Feher,V.A., Y-L. Tzeng, J.A. Hoch and John Cavanagh. 1998. Identification of communication networks in Spo0F: a model for phosphorylation-induced conformational change and implications for activation of multiple domain bacterial response regulators. FEBS Letters 425:1-6
Barrett, J.F., R.M. Goldschmidt, L.E. Lawrence, B. Foleno, R. Chen, J.P. Demers, S. Johnson, R. Kanojia, J. Fernandez, J. Bernstein, L. Licata, A. Donetz, S. Huang, D.J. Hlasta, M.J. Macielag, K. Ohemeng, R. Frechette, M.B. Frosco, D.H. Klaubert, J.M. Whiteley, L. Wang and J.A. Hoch. 1998. Novel antibacterial agents that inhibit two-component signal transduction systems. Proc.Natl.Acad.Sci.USA 95(9):5317-5322
Manson, M.D., J.P. Armitage, J.A. Hoch and R.M. MacNab. 1998. Bacterial locomotion and signal transduction. J. Bacteriol. 180(5):1009-1022
Demina, A., Varughese, K.I., Barbot, J., Forman, K., Beutler, E. 1998. Six previously undescribed pyruvate kinase mutations causing enzyme deficiency. Blood 92(2):647-652
Zapf, J., Madusudan, C.E. Grimshaw, J.A. Hoch, K.I. Varughese and J.M. Whiteley. 1998. A source of response regulator autophosphatase activity: the critical role of a residue adjacent to the Spo0F autophosphorylation active-site. Biochemistry 37(21):7725-7732
Grimshaw, C.E., C.G. Hanstein, M.A. Strauch, D. Burbulys, S. Huang, L. Wang, J.A. Hoch and J.M. Whiteley. 1998. Synergistic kinetic interactions between components of the phosphorelay controlling sporulation in Bacillus subtilis. Biochemistry 37(5):1365-1375
Celikel, R., Varughese, K.I., Madhusudan, Yoshioka, A., Ware, J., Ruggeri, Z.M. 1998. Crystal structure of the von Willebrand factor A1 domain in complex with function blocking NMC-4 Fab. Nature Struc. Biol. 5:189-194.
Perego, M. 1998. Kinase-phosphatase competition regulates Bacillus subtilis development. Trends in Microbiol. 6(9):366-370
Strauch, M.A. and J.A. Hoch. 1997. Spores, Sporulation. In T.E. Creighton (ed.) The Encyclopedia of Molecular Biology. John Wiley & Sons.
Hoch, J.A. and R. Losick. 1997. Panspermia, spores and the Bacillus subtilis genome. Nature. 390(6657):237-238
Reizer, J., A. Reizer, M. Perego and M.H. Saier, Jr. 1997. Characterization of a family of bacterial response regulator aspartyl-phosphate (RAP) phosphatases. Microb. & Compar. Genom. 2(2):103-111
Celikel, R., Madhusudan, Varughese, K.I., Shima, M., Yoshioka, A., Ware, J., Ruggeri, Z.M. 1997. Crystal structure on NMC-4 Fab anti-von Willebrand Factor A1 domain. Blood Cells, Molecules & Dis. 23(7):123-134
Dartois,V., T. Djavakhishvili and J.A. Hoch. 1997. KapB is a lipoprotein required for KinB signal transduction and activation of the phosphorelay to sporulation in Bacillus subtilis. Mol.Microbiol. 26(5):1097-1108
Balakrishnan, R., Ramasubbu, N., Varughese, K.I., Parthasarathy, R. 1997. Crystal structures of the copper and nickel complexes of Rnase A: Metal-induced interprotein interactions and identification of a novel copper binding motif. Proc.Natl.Adac.Sci.USA. 94(18):9620-9625
Madusudan, J.W. Zapf, J.A. Hoch, J.M. Whiteley, N.H. Xuong and K.I. Varughese. 1997. A response regulatory protein with the site of phosphorylation blocked by an arginine interaction: crystal structure of Spo0F from Bacillus subtilis. Biochemistry. 36(42):12739-12745
Perego, M. 1997. A peptide export-import control circuit modulating bacterial development regulates protein phosphatases of the phosphorelay. Proc. Natl. Acad. Sci. USA. 94(16):8612-8617
Wang, L., R. Grau, M. Perego and J.A. Hoch. 1997. A novel histidine kinase inhibitor regulating development in Bacillus subtilis. Genes & Dev. 11:2569-2579
Tzeng, Y.-L. and J.A. Hoch. 1997. Molecular recognition in signal transduction: the interaction surfaces of the Spo0F response regulator with its cognate phosphorelay proteins revealed by alanine scanning mutagenesis. J.Mol.Biol. 272(2):200-212
Zhou, X.Z., Madusudan, J.M. Whiteley, J.A. Hoch and K.I. Varughese. 1997. Purification and preliminary crystallographic studies on the sporulation response regulatory phosphotransferase protein, Spo0B, from Bacillus subtilis. Proteins 27(4):597-600
Glaser, P. M.E. Sharpe, M. Perego, K. Ohlsen, and J. Errington. 1997. Dynamic, mitotic-like behavior of a bacterial protein required for accurate chromosome partitioning. Genes & Dev. 11:1160-1168
Feher, V.A., J.W. Zapf, J.A. Hoch, J.M. Whiteley, L.P. McIntosh, M. Rance, N.J. Skelton, F.W. Dahlquist and J. Cavanagh. 1997. High resolution solution structure and dynamics of Bacillus subtilis response regulator, Spo0F: implications for phosphorylation and molecular recognition. Biochemistry 36(33):10015-10025
Dartois, V., J. Liu and J.A. Hoch. 1997. Alterations in the flow of one-carbon units affect KinB-dependent sporulation in Bacillus subtilis. Mol.Microbiol. 25(1):39-5